Frequent RFLP recognised by an anonymous sequence localise to 11q13 – Q14[D11S. 3.7.(E79)]

An anonymous single copy chromosome 21 probe, DS21D2, associated with a frequent RFLP.

SOURCE/DESCRIPTION An EcoRI 4.0 kb single copy fragment insert into XgtWES Xb isolated from the chromosome. 21 flow-sorted library of Krumlauf et al (1982). POLYMORPHISM TaqI (T-J-CGA) identifies 2 invariant bands and a simple 2 allele polymorphism with a band at 8.4 and/or 5.8 kb. FREQUENCY St-ndled 10 unrelated individuals. 8.4 kb allele (Al) 0.65 5.8 kb allele (A2) 0.35 CHROMOSOMAL LOCALISAT...

Human gastrin (GAS) RFLP recognised by digestion with BamHI or EcoRI.

SOURCE AUD DESCRIPTION OF CLONE: The human gastrin gene cDNA insert in clone pHG529 (1) is 463 bp in length. This excludes the poly (dC) tail* and includes 8 nucleotides of 5' untranslated region, the entire coding region of 303 nucleotides, a 3 1 untranslated region of 102 nucleotides and a poly (A) track of 50 residues.

A frequent Hindlll RFLP of the human fibrorrertin gene ( FNl )

SOURCE/DESCRIPTION: The probe used is a Hindlll/BamHI fragment of 1.4kb from the fibronectin recombinant, pFH6 (Kornblihtt, A.R. et al.). POLYMORPHISM: Hindlll (A/AGCTT) (New England, Bio-Labs, FRG) detects invariant bands at 4.75 and 1.7kb in addition to a simple two-allele polymorphism defined by the presence of a band of 7.1kb (F2) alternatively with two bands of 3.7 and 3.4kb (Fl) (Figure 1...

RFLP identified by the anonymous DNA segment FZ IV E11 at 1p23 [HGM9 no.D1S87].

A PPY cDNA clone identifies a frequent RFLP.

SOURCE/DESCRIPTION: A pancreatic polypeptide cDNA clone (465 base pair Insert 1n the vector pBR322) was isolated from a library created from human pancreatic neoplasm RNA. This cDNA clone spans a 95 amino acid sequence in the coding region with a 29 amino add amino terminus leader sequence, a three amino acid connecting sequence and a 27 amino add carboxyl terminus (Takeuchi and Yamada, 1985). ...